Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Feb;118(2):506-14.
doi: 10.1111/jam.12686. Epub 2014 Dec 22.

Cell-bound Exopolysaccharides of Lactobacillus Brevis KB290 Enhance Cytotoxic Activity of Mouse Splenocytes

Affiliations
Free article

Cell-bound Exopolysaccharides of Lactobacillus Brevis KB290 Enhance Cytotoxic Activity of Mouse Splenocytes

E Sasaki et al. J Appl Microbiol. .
Free article

Abstract

Aims: This study aimed to identify the main active component of Lactobacillus brevis KB290 (KB290) that is responsible for enhanced cell-mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate innate immune responses as affecting on cell-mediated cytotoxic activity of mouse splenocytes.

Methods and results: We used live KB290, heat-killed KB290, a derivative strain (Lact. brevis KB392) with different amounts of cell-bound exopolysaccharide (EPS-b), and a crude extract of EPS-b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10(10) CFU live KB290, 10(10) CFU live KB392, 15 mg heat-killed KB290 or 600 μg crude extract of EPS-b for 1 day. Live KB290 (P < 0.01), heat-killed KB290 (P < 0.05) and crude EPS-b at 600 μg (P < 0.05) per mouse significantly enhanced cytotoxic activity; however, live KB392 had no effect.

Conclusions: Both live and heat-killed KB290 and crude EPS-b significantly enhanced cytotoxic activity of mouse splenocytes.

Significance and impact of the study: We demonstrated that EPS-b produced by KB290 has a critical role in enhancing cell-mediated cytotoxic activity in mouse spleen.

Keywords: Lactobacillus brevis KB290; Lactobacillus brevis KB392; cell-bound exopolysaccharides; cell-mediated cytotoxicity; heat-killed KB290.

Similar articles

See all similar articles

Cited by 1 article

Publication types

Substances

LinkOut - more resources

Feedback