As one of the most important components copying DNA molecules in the PCR system, Taq DNA polymerase has a high processivity, however, lower persistence when compared to other polymerases. Studies for the enhancement of stability of Taq DNA polymerase is of great importance. The present study describes the integration of PCR application of cross-linked Taq DNA polymerase enzyme in a nanochamber using a ruthenium based MATyr-Ru-(bipyr)2)-MATyr monomer hapten prepared by photosensitive microemulsion polymerization technique. The conjugation and cross-linking have achieved using our previously invented Aminoacid (monomer) Decorated and Light Underpining Conjugation Approach (ANADOLUCA) method. Microemulsion polymerization media has prepared by dispersing PVA in deionized water. The nano enzyme could be easily prepared at room temperature, in daylight and under nitrogen atmosphere using ruthenium based photosensitive cross-linking agents. The nano copy machine particles (nano Taq DNA polymerase) are very stable against more acidic or more basic conditions, high temperatures and could be reusable in PCR analysis for many times without any deformation in their structures.
Keywords: ANADOLUCA method; DNA replication; PCR technology; nanocopiers; nanoenzymes.
© 2014 American Institute of Chemical Engineers.