Light-emitting diode therapy in exercise-trained mice increases muscle performance, cytochrome c oxidase activity, ATP and cell proliferation

J Biophotonics. 2015 Sep;8(9):740-54. doi: 10.1002/jbio.201400087. Epub 2014 Nov 6.


Light-emitting diode therapy (LEDT) applied over the leg, gluteus and lower-back muscles of mice using a LED cluster (630 nm and 850 nm, 80 mW/cm(2) , 7.2 J/cm(2) ) increased muscle performance (repetitive climbing of a ladder carrying a water-filled tube attached to the tail), ATP and mitochondrial metabolism; oxidative stress and proliferative myocyte markers in mice subjected to acute and progressive strength training. Six bi-daily training sessions LEDT-After and LEDT-Before-After regimens more than doubled muscle performance and increased ATP more than tenfold. The effectiveness of LEDT on improving muscle performance and recovery suggest applicability for high performance sports and in training programs. Positioning of the mice and light-emitting diode therapy (LEDT) applied on mouse legs, gluteus and lower-back muscles without contact.

Keywords: ATP; LEDT; LLLT; muscle performance; muscle recovery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Biomarkers / metabolism
  • Catalase / metabolism
  • Cell Proliferation / radiation effects
  • Electron Transport Complex IV / metabolism*
  • Glycogen / metabolism
  • Lipid Peroxidation / radiation effects
  • Low-Level Light Therapy*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Muscle Strength / radiation effects
  • Muscles / cytology
  • Muscles / metabolism
  • Muscles / physiology*
  • Muscles / radiation effects*
  • Oxidative Stress / radiation effects
  • Physical Conditioning, Animal*
  • Protein Carbonylation / radiation effects
  • Weight-Bearing


  • Biomarkers
  • Adenosine Triphosphate
  • Glycogen
  • Catalase
  • Electron Transport Complex IV