REBASE--a database for DNA restriction and modification: enzymes, genes and genomes

Nucleic Acids Res. 2015 Jan;43(Database issue):D298-9. doi: 10.1093/nar/gku1046. Epub 2014 Nov 5.


REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data. All genomes that are completely sequenced are analyzed for RM system components, and with the advent of PacBio sequencing, the recognition sequences of DNA methyltransferases (MTases) are appearing rapidly. Thus, Type I and Type III systems can now be characterized in terms of recognition specificity merely by DNA sequencing. The contents of REBASE may be browsed from the web and selected compilations can be downloaded by FTP ( Monthly updates are also available via email.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Modification Methylases / chemistry
  • DNA Modification Methylases / genetics
  • DNA Modification Methylases / metabolism*
  • DNA Restriction Enzymes / chemistry
  • DNA Restriction Enzymes / genetics
  • DNA Restriction Enzymes / metabolism*
  • Databases, Genetic*
  • Genes
  • Genomics
  • Internet
  • Sequence Analysis, DNA
  • Software


  • DNA Modification Methylases
  • DNA Restriction Enzymes