Nrf2 and redox status in prediabetic and diabetic patients

Int J Mol Sci. 2014 Nov 6;15(11):20290-305. doi: 10.3390/ijms151120290.

Abstract

The redox status associated with nuclear factor erythroid 2-related factor-2 (Nrf2) was evaluated in prediabetic and diabetic subjects. Total antioxidant status (TAS) in plasma and erythrocytes, glutathione (GSH) and malondialdehyde (MDA) content and activity of antioxidant enzymes were measured as redox status markers in 259 controls, 111 prediabetics and 186 diabetic type 2 subjects. Nrf2 was measured in nuclear extract fractions from peripheral blood mononuclear cells (PBMC). Nrf2 levels were lower in prediabetic and diabetic patients. TAS, GSH and activity of glutamate cysteine ligase were lower in diabetic subjects. An increase of MDA and superoxide dismutase activity was found in diabetic subjects. These results suggest that low levels of Nrf2 are involved in the development of oxidative stress and redox status disbalance in diabetic patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Age Factors
  • Antioxidant Response Elements / genetics
  • Antioxidants / metabolism
  • Biomarkers / metabolism
  • Case-Control Studies
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / enzymology
  • Diabetes Mellitus, Type 2 / metabolism*
  • Female
  • Glycated Hemoglobin A / metabolism
  • Hospitalization
  • Humans
  • Male
  • Middle Aged
  • NF-E2-Related Factor 2 / metabolism*
  • Oxidation-Reduction
  • Prediabetic State / blood
  • Prediabetic State / enzymology
  • Prediabetic State / metabolism*
  • Protein Binding

Substances

  • Antioxidants
  • Biomarkers
  • Glycated Hemoglobin A
  • NF-E2-Related Factor 2