Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies

Am J Physiol Cell Physiol. 2015 Feb 1;308(3):C209-19. doi: 10.1152/ajpcell.00263.2014. Epub 2014 Nov 12.


Production and isolation of forebrain interneuron progenitors are essential for understanding cortical development and developing cell-based therapies for developmental and neurodegenerative disorders. We demonstrate production of a population of putative calretinin-positive bipolar interneurons that express markers consistent with caudal ganglionic eminence identities. Using serum-free embryoid bodies (SFEBs) generated from human inducible pluripotent stem cells (iPSCs), we demonstrate that these interneuron progenitors exhibit morphological, immunocytochemical, and electrophysiological hallmarks of developing cortical interneurons. Finally, we develop a fluorescence-activated cell-sorting strategy to isolate interneuron progenitors from SFEBs to allow development of a purified population of these cells. Identification of this critical neuronal cell type within iPSC-derived SFEBs is an important and novel step in describing cortical development in this iPSC preparation.

Keywords: calretinin; cortex; inducible pluripotent stem cell; interneuron; neuron progenitor; serum-free embryoid body.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Cerebral Cortex / cytology*
  • Cerebral Cortex / physiology*
  • Embryoid Bodies / physiology*
  • Fibroblasts / physiology
  • Humans
  • Induced Pluripotent Stem Cells / physiology*
  • Interneurons / physiology*
  • Mice
  • Mice, Knockout