FlyBase: introduction of the Drosophila melanogaster Release 6 reference genome assembly and large-scale migration of genome annotations

Nucleic Acids Res. 2015 Jan;43(Database issue):D690-7. doi: 10.1093/nar/gku1099. Epub 2014 Nov 14.


Release 6, the latest reference genome assembly of the fruit fly Drosophila melanogaster, was released by the Berkeley Drosophila Genome Project in 2014; it replaces their previous Release 5 genome assembly, which had been the reference genome assembly for over 7 years. With the enormous amount of information now attached to the D. melanogaster genome in public repositories and individual laboratories, the replacement of the previous assembly by the new one is a major event requiring careful migration of annotations and genome-anchored data to the new, improved assembly. In this report, we describe the attributes of the new Release 6 reference genome assembly, the migration of FlyBase genome annotations to this new assembly, how genome features on this new assembly can be viewed in FlyBase ( and how users can convert coordinates for their own data to the corresponding Release 6 coordinates.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Databases, Genetic*
  • Drosophila melanogaster / genetics*
  • Genome, Insect*
  • Genomics / standards
  • High-Throughput Nucleotide Sequencing
  • Internet
  • Models, Genetic
  • Molecular Sequence Annotation*
  • Molecular Sequence Data
  • Reference Standards
  • Sequence Alignment
  • Software

Associated data

  • GENBANK/AE013599
  • GENBANK/AE014134
  • GENBANK/AE014135
  • GENBANK/AE014296
  • GENBANK/AE014297
  • GENBANK/CP007106
  • GENBANK/U37541
  • RefSeq/NC_001709
  • RefSeq/NC_004353
  • RefSeq/NC_004354
  • RefSeq/NC_024512
  • RefSeq/NT_033777
  • RefSeq/NT_033778
  • RefSeq/NT_033779
  • RefSeq/NT_037436