Abstract
Biosynthetic incorporation of β-(1-azulenyl)-L-alanine, an isostere of tryptophan, is reported using a tryptophan auxotroph expression host. The azulene moiety introduced this way in proteins features many attractive spectroscopic properties, particularly suitable for in vivo studies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alanine / metabolism*
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Azulenes / metabolism*
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / genetics
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Lactococcus lactis / genetics
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Lactococcus lactis / metabolism*
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Protein Biosynthesis*
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Tryptophan / metabolism
Substances
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Azulenes
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Bacterial Proteins
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azulene
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Tryptophan
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Alanine