Biosynthetic incorporation of the azulene moiety in proteins with high efficiency

Amino Acids. 2015 Jan;47(1):213-6. doi: 10.1007/s00726-014-1870-4. Epub 2014 Nov 16.

Abstract

Biosynthetic incorporation of β-(1-azulenyl)-L-alanine, an isostere of tryptophan, is reported using a tryptophan auxotroph expression host. The azulene moiety introduced this way in proteins features many attractive spectroscopic properties, particularly suitable for in vivo studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / metabolism*
  • Azulenes / metabolism*
  • Bacterial Proteins / biosynthesis*
  • Bacterial Proteins / genetics
  • Lactococcus lactis / genetics
  • Lactococcus lactis / metabolism*
  • Protein Biosynthesis*
  • Tryptophan / metabolism

Substances

  • Azulenes
  • Bacterial Proteins
  • azulene
  • Tryptophan
  • Alanine