In vitro pollen viability and pollen germination in cherry laurel (Prunus laurocerasus L.)

ScientificWorldJournal. 2014;2014:657123. doi: 10.1155/2014/657123. Epub 2014 Oct 22.

Abstract

Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r(2) = 0.0614 and r(2) = 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

MeSH terms

  • Cell Survival / drug effects
  • Culture Media / chemistry
  • Culture Techniques
  • Genotype
  • Germination / drug effects*
  • Germination / physiology
  • Iodine Compounds
  • Pollen / cytology
  • Pollen / drug effects*
  • Pollen / physiology
  • Prunus / physiology
  • Staining and Labeling / methods
  • Sucrose / pharmacology*
  • Tetrazolium Salts

Substances

  • Culture Media
  • Iodine Compounds
  • Tetrazolium Salts
  • iodine potassium iodide
  • Sucrose
  • triphenyltetrazolium