Aminobacterium Thunnarium Sp. Nov., a Mesophilic, Amino Acid-Degrading Bacterium Isolated From an Anaerobic Sludge Digester, Pertaining to the Phylum Synergistetes

Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):609-614. doi: 10.1099/ijs.0.068965-0. Epub 2014 Nov 18.

Abstract

A new Gram-staining-positive, non-sporulating, mesophilic, amino acid-degrading anaerobic bacterium, designated strain OTA 102(T), was isolated from an anaerobic sequencing batch reactor treating wastewater from cooking tuna. The cells were curved rods (0.6-2.5×0.5 µm) and occurred singly or in pairs. The strain was motile by means of one lateral flagellum. Strain OTA 102(T) grew at temperatures between 30 and 45 °C (optimum 40 °C), between pH 6.0 and 8.4 (optimum pH 7.2) and NaCl concentrations between 1 and 5 % (optimum 2 %, w/v). Strain OTA 102(T) required yeast extract for growth. Serine, threonine, glycine, cysteine, citrate, fumarate, α-ketoglutarate and pyruvate were fermented. When co-cultured with Methanobacterium formicicum as the hydrogen scavenger, strain OTA 102(T) oxidized alanine, valine, leucine, isoleucine, aspartate, tyrosine, methionine, histidine and asparagine. The genomic DNA G+C content of strain OTA 102(T) was 41.7 mol%. The main fatty acid was iso-C15 : 0. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain OTA 102(T) was related to Aminobacterium colombiense and Aminobacterium mobile (95.5 and 95.2 % similarity, respectively), of the phylum Synergistetes. On the basis of phylogenetic, genetic and physiological characteristics, strain OTA 102(T) is proposed to represent a novel species of the genus Aminobacterium, Aminobacterium thunnarium sp. nov. The type strain is OTA 102(T) ( = DSM 27500(T) = JCM 19320(T)).

MeSH terms

  • Amino Acids / metabolism*
  • Bacteria, Anaerobic / classification*
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / isolation & purification
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Fermentation
  • Gram-Positive Bacteria / classification*
  • Gram-Positive Bacteria / genetics
  • Gram-Positive Bacteria / isolation & purification
  • Ketoglutaric Acids
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sewage / microbiology*
  • Tunisia

Substances

  • Amino Acids
  • DNA, Bacterial
  • Fatty Acids
  • Ketoglutaric Acids
  • RNA, Ribosomal, 16S
  • Sewage

Associated data

  • GENBANK/KJ159211