Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Nov 19;14:278.
doi: 10.1186/s12866-014-0278-3.

Production of Potent Antimicrobial Agent by Actinomycete, Streptomyces Sannanensis Strain SU118 Isolated From Phoomdi in Loktak Lake of Manipur, India

Affiliations
Free PMC article

Production of Potent Antimicrobial Agent by Actinomycete, Streptomyces Sannanensis Strain SU118 Isolated From Phoomdi in Loktak Lake of Manipur, India

Laishram Shantikumar Singh et al. BMC Microbiol. .
Free PMC article

Abstract

Background: Actinomycetes have provided a wealth of bioactive secondary metabolites with interesting activities such as antimicrobial, antiviral and anticancer. The study aims at isolation, characterization and the antimicrobial potentiality of Streptomyces sannanensis SU118 obtained from Phoomdi, a unique habitat of Loktak Lake of Manipur, India.

Results: An actinomycete strain isolated from Phoomdi soil of Loktak Lake of Manipur, India was identified as Streptomyces sannanensis SU118. It is a Gram-positive filamentous bacterium which exhibits antimicrobial activity only against Gram-positive bacteria, while Gram-negative organisms were not affected. Glucose Soyabean meal broth was found to be the suitable medium for antibiotic production at 28°C for seven days of incubation. The antimicrobial agent produced by the strain was extracted with ethyl acetate as solvent and purified by thin layer chromatography. Screening and bioassay - guided fractionation of the ethyl acetate extract from the culture filtrate led to the isolation of an active potential compound (R f value 0.56) with λmax 275.0 nm which has got the lowest minimum inhibitory concentration (0.5 μg/ml) against Staphylococcus aureus MTCC 96 and Staphylococcus aureus (clinical isolate), whereas highest (3.0 μg/ml) was recorded against Mycobacterium smegmatis MTCC 6 and Bacillus circulans MTCC 8074.

Conclusion: This study has therefore uncovered the potential of exploring virgin untapped habitats in the Indo-Burma biodiversity hot spot region as reservoir of promising antimicrobial metabolite producer. These results highlighted the scope for further characterization of the metabolite and could be a candidate in the generation of new antimicrobial agents.

Figures

Figure 1
Figure 1
Phylogenetic tree based on 16S rRNA gene sequences from the strain SU118 and other Streptomyces species.
Figure 2
Figure 2
Effect of various culture media on growth and antimicrobial agent production by Streptomyces sannanensis SU118 (SCB- Starch casein broth; AB- Actinomycete broth; NB- Nutrient broth; GSB- Glucose soyabean meal broth).
Figure 3
Figure 3
Effect of incubation temperature on growth and antimicrobial agent production by Streptomyces sannanensis SU118.
Figure 4
Figure 4
Effect of pH on growth and antimicrobial agent production by Streptomyces sannanensis SU118.
Figure 5
Figure 5
Effect of incubation period on growth and antimicrobial agent production by Streptomyces sannanensis SU118.
Figure 6
Figure 6
Specific rate of antimicrobial agent production (qp) by Streptomyces sannanensis SU118 .
Figure 7
Figure 7
Thin layer chromatography plate showing the separation of bioactive compound (fraction B) obtained from Streptomyces sannanensis SU118.

Similar articles

See all similar articles

Cited by 11 articles

See all "Cited by" articles

References

    1. Takahashi Y. Exploitation of new microbial resources for bioactive compounds and discovery of new actinomycetes. Actinomycetology. 2004;18:54–61. doi: 10.3209/saj.18_54. - DOI
    1. Meena B, Rajan LA, Vinithkumar NV, Kirubagaran R. Novel marine actinobacteria from emerald Andaman & Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts. BMC Microbiol. 2013;13:145–161. doi: 10.1186/1471-2180-13-145. - DOI - PMC - PubMed
    1. Zin NM, Sarmin NIM, Ghadin N, Basri DF, Sidik NM, Hess WM, Strobel GA. Bioactive endophytic streptomycetes from the Malay Peninsula. FEMS Microbiol Lett. 2007;274:83–88. doi: 10.1111/j.1574-6968.2007.00819.x. - DOI - PubMed
    1. Berdy J. Bioactive microbial metabolites; a personal view. J Antibiot. 2005;58(1):1–26. doi: 10.1038/ja.2005.1. - DOI - PubMed
    1. Goshi K, Uchida T, Lezhava A, Yamasaki M, Hiratsu K, Shinkawa H, Kinashi H. Cloning and analysis of the telomere and terminal inverted repeat of the linear chromosome of Streptomyces griseus. J Bacteriol. 2002;184:3411–3415. doi: 10.1128/JB.184.12.3411-3415.2002. - DOI - PMC - PubMed

Publication types

MeSH terms

Associated data

Feedback