OMIP-025: evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining

Cytometry A. 2015 Apr;87(4):289-92. doi: 10.1002/cyto.a.22590. Epub 2014 Nov 18.


This panel was developed to assess antigen-specific T cells using peptide pools to various antigens of interest, although other types of antigens such as recombinant proteins or whole pathogens could be considered using different stimulation times. In addition to multiple functional markers, the panel includes differentiation markers and markers to assess follicular helper T cells and NK cells (Table 1). It was optimized using cryopreserved peripheral blood mononuclear cells (PBMC) from human immunodeficiency virus (HIV) uninfected and HIV infected adults with known cytomegalovirus (CMV) responses and it underwent assay qualification. The panel is being used to evaluate the responses to HIV and malaria vaccine candidates in adults and children from different geographic areas.

MeSH terms

  • AIDS Vaccines / immunology
  • Adult
  • Antigens, CD / immunology
  • Biomarkers / analysis
  • Child
  • Cytokines / analysis*
  • Cytomegalovirus / immunology
  • Flow Cytometry / methods*
  • HIV Infections / immunology
  • Humans
  • Immunologic Memory / immunology
  • Interferon-gamma / immunology
  • Interleukin-2 / immunology
  • Killer Cells, Natural / immunology*
  • Leukocytes, Mononuclear / immunology
  • Malaria Vaccines / immunology
  • Phenotype
  • Staining and Labeling / methods*
  • T-Lymphocytes, Helper-Inducer / immunology*
  • Tumor Necrosis Factor-alpha / immunology


  • AIDS Vaccines
  • Antigens, CD
  • Biomarkers
  • Cytokines
  • IL2 protein, human
  • Interleukin-2
  • Malaria Vaccines
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma