Host, Pathogen, and Environmental Characteristics Predict White-Nose Syndrome Mortality in Captive Little Brown Myotis (Myotis Lucifugus)

PLoS One. 2014 Nov 19;9(11):e112502. doi: 10.1371/journal.pone.0112502. eCollection 2014.

Abstract

An estimated 5.7 million or more bats died in North America between 2006 and 2012 due to infection with the fungus Pseudogymnoascus destructans (Pd) that causes white-nose syndrome (WNS) during hibernation. The behavioral and physiological changes associated with hibernation leave bats vulnerable to WNS, but the persistence of bats within the contaminated regions of North America suggests that survival might vary predictably among individuals or in relation to environmental conditions. To investigate variables influencing WNS mortality, we conducted a captive study of 147 little brown myotis (Myotis lucifugus) inoculated with 0, 500, 5000, 50,000, or 500,000 Pd conidia and hibernated for five months at either 4 or 10°C. We found that female bats were significantly more likely to survive hibernation, as were bats hibernated at 4°C, and bats with greater body condition at the start of hibernation. Although all bats inoculated with Pd exhibited shorter torpor bouts compared to controls, a characteristic of WNS, only bats inoculated with 500 conidia had significantly lower survival odds compared to controls. These data show that host and environmental characteristics are significant predictors of WNS mortality, and that exposure to up to 500 conidia is sufficient to cause a fatal infection. These results also illustrate a need to quantify dynamics of Pd exposure in free-ranging bats, as dynamics of WNS produced in captive studies inoculating bats with several hundred thousand conidia may differ from those in the wild.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Ascomycota / genetics
  • Ascomycota / physiology*
  • Chiroptera / microbiology*
  • Chiroptera / physiology
  • DNA, Fungal / analysis
  • Environment*
  • Female
  • Hibernation
  • Host-Pathogen Interactions*
  • Male
  • Mycoses / mortality*
  • Sex Characteristics
  • Time Factors

Substances

  • DNA, Fungal

Grant support

Funding for this project was provided by the United States Fish and Wildlife Service grant F12AP01210 (DMR and KAF) and the Woodtiger Foundation (DMR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.