Epstein-Barr virus BZLF1 trans-activator specifically binds to a consensus AP-1 site and is related to c-fos

EMBO J. 1989 Jan;8(1):127-32. doi: 10.1002/j.1460-2075.1989.tb03356.x.


Two regions of the Epstein-Barr virus BZLF1 trans-activator protein have sequence similarity to the c-fos protein. Part of the similarity corresponds to the region of c-fos which is similar to the DNA binding domain of c-jun and GCN-4. The structure of the exon which contains this region in c-fos and BZLF1 is also highly conserved between the two genes. Complete BZLF1 protein and a C terminal fragment were prepared either as purified fusion proteins or by in vitro translation from a BZLF1 cDNA. Gel retardation and DNase footprinting assays using these proteins show that BZLF1 is a sequence specific DNA binding protein capable of binding to a target sequence which contains a consensus AP-1 site.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Antigens / genetics
  • Binding Sites
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / genetics
  • Gene Products, tat
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / metabolism*
  • Molecular Sequence Data
  • Protein Kinases*
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*


  • Antigens
  • DNA-Binding Proteins
  • Fungal Proteins
  • Gene Products, tat
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Viral Proteins
  • Protein Kinases