A combination of glucosyl hesperidin and caffeine exhibits an anti-obesity effect by inhibition of hepatic lipogenesis in mice

Phytother Res. 2015 Feb;29(2):310-6. doi: 10.1002/ptr.5258. Epub 2014 Nov 20.


To develop an anti-obesity agent, we examined the combination effect of glucosyl hesperidin (G-hesperidin) and caffeine on obesity in mice. High-fat diet-induced obese KK mice were fed a low-fat diet with or without G-hesperidin, caffeine, or their combination for 2 weeks. Decreases in body weight and significantly lower adipose tissue weight were observed in the combination-fed mice but not in the G-hesperidin-fed or caffeine-fed mice. DNA microarray analysis of mouse liver suggested that the feeding of G-hesperidin + caffeine was associated with lower lipogenesis. Therefore, we examined the anti-lipogenic effect of G-hesperidin + caffeine in fasted-refed KK mice. Hepatic triglyceride levels were significantly lower in the mice fed G-hesperidin + caffeine during the refeeding period but not in the mice fed each alone. In addition, hepatic expressions of genes related to lipogenesis, such as sterol regulatory element-binding protein-1c or fatty acid synthase, were significantly lower in the mice fed G-hesperidin + caffeine compared with that in the control mice. These results suggested that G-hesperidin + caffeine is effective for controlling obesity partly by the inhibition of hepatic lipogenesis.

Keywords: anti-obesity effect; caffeine; glucosyl hesperidin; lipogenesis; liver.

MeSH terms

  • Adipogenesis / drug effects
  • Adipose Tissue / drug effects
  • Animals
  • Anti-Obesity Agents / pharmacology*
  • Body Weight / drug effects
  • Caffeine / pharmacology*
  • Diet, High-Fat
  • Fatty Acid Synthases / metabolism
  • Glucosides / pharmacology*
  • Hesperidin / analogs & derivatives*
  • Hesperidin / pharmacology
  • Lipids / blood
  • Lipogenesis / drug effects*
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Obese
  • Obesity / metabolism*
  • Sterol Regulatory Element Binding Protein 1 / metabolism


  • Anti-Obesity Agents
  • Glucosides
  • Lipids
  • Sterol Regulatory Element Binding Protein 1
  • Caffeine
  • glucosyl hesperidin
  • Hesperidin
  • Fatty Acid Synthases