Molecular cloning and chromosomal localization of a novel Drosophila protein phosphatase

FEBS Lett. 1989 Apr 24;247(2):391-5. doi: 10.1016/0014-5793(89)81377-8.

Abstract

A 1.0 kilobase cDNA coding for the complete amino acid sequence of a putative protein phosphatase (314 amino acid residues, molecular mass 36 kDa) has been isolated from a Drosophila head cDNA library. The cDNA hybridises to a single site on the right arm of the second chromosome at cytological position 55A1-3. The deduced sequence of the protein, designated protein phosphatase-Y, is homologous to the catalytic subunits of Drosophila and rabbit protein phosphatase-1 alpha (64 and 59% identity, respectively) and rabbit protein phosphatase-2A (39% identity). These and other comparisons demonstrate that this novel enzyme is not the Drosophila counterpart of mammalian protein phosphatases 1, 2A, 2B, 2C or X.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Cloning, Molecular*
  • DNA / genetics
  • DNA / isolation & purification
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Phosphoprotein Phosphatases / genetics*
  • Protein Phosphatase 1
  • Protein Phosphatase 2
  • Rabbits
  • Sequence Homology, Nucleic Acid

Substances

  • DNA
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1
  • Protein Phosphatase 2

Associated data

  • GENBANK/Y07510