Abstract
Receptors for insulin and insulin-like growth factor-1 (IGF-1) have been demonstrated on activated T-lymphocytes. The question is whether receptors for insulin or IGF-1 have any function in these cells. In this study we demonstrate that the concentration of IGF-1 in commercial samples of fetal calf serum is about 70 times that of insulin. Moreover, antibodies binding IGF-1 reduce responses of human peripheral blood mononuclear cells to PHA by about 50%, whereas antibodies to insulin have no demonstrable effect. These observations suggest that binding of IGF-1 to specific receptors contributes to the proliferative responses of activated T-lymphocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / immunology
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Cattle / blood
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Cell Division / drug effects
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Fetal Blood / physiology
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Humans
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Insulin-Like Growth Factor I / immunology
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Insulin-Like Growth Factor I / isolation & purification
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Insulin-Like Growth Factor I / pharmacology*
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Lymphocyte Activation / drug effects*
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Phytohemagglutinins / pharmacology*
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Receptors, Cell Surface / physiology
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Receptors, Somatomedin
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Recombinant Proteins / pharmacology
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Somatomedins / pharmacology*
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Stimulation, Chemical
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects*
Substances
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Antibodies
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Phytohemagglutinins
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Receptors, Cell Surface
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Receptors, Somatomedin
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Recombinant Proteins
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Somatomedins
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Insulin-Like Growth Factor I