Human NAT10 is an ATP-dependent RNA acetyltransferase responsible for N4-acetylcytidine formation in 18 S ribosomal RNA (rRNA)

J Biol Chem. 2014 Dec 26;289(52):35724-30. doi: 10.1074/jbc.C114.602698. Epub 2014 Nov 19.


Human N-acetyltransferase 10 (NAT10) is known to be a lysine acetyltransferase that targets microtubules and histones and plays an important role in cell division. NAT10 is highly expressed in malignant tumors, and is also a promising target for therapies against laminopathies and premature aging. Here we report that NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N(4)-acetylcytidine (ac(4)C) at position 1842 in the terminal helix of mammalian 18 S rRNA. RNAi-mediated knockdown of NAT10 resulted in growth retardation of human cells, and this was accompanied by high-level accumulation of the 30 S precursor of 18 S rRNA, suggesting that ac(4)C1842 formation catalyzed by NAT10 is involved in rRNA processing and ribosome biogenesis.

Keywords: Acetyl Coenzyme A (Acetyl-CoA); Acetyltransferase; RNA Modification; Ribosomal RNA Processing (rRNA Processing); Ribosome Assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Base Sequence
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • N-Terminal Acetyltransferase E / physiology*
  • N-Terminal Acetyltransferases
  • Nucleic Acid Conformation
  • RNA Processing, Post-Transcriptional
  • RNA, Ribosomal, 18S / metabolism*


  • RNA, Ribosomal, 18S
  • N-Terminal Acetyltransferase E
  • N-Terminal Acetyltransferases
  • NAT10 protein, human