Bovine myeloperoxidase and lactoperoxidase each contain a high affinity site for calcium

Biochem Biophys Res Commun. 1989 Apr 28;160(2):897-902. doi: 10.1016/0006-291x(89)92519-9.

Abstract

Both bovine myeloperoxidase and lactoperoxidase contain one calcium per iron with no other metal present in significant amount. Calcium is bound with high affinity and is removed upon exposure to 6 M guanidine hydrochloride/EGTA which results in precipitation of protein. Computer amino acid sequence analyses of human myeloperoxidase reveal two plausible calcium binding sites. This is the first evidence for the presence of calcium in these peroxidases.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Calcium / metabolism*
  • Calcium-Binding Proteins / metabolism*
  • Cattle
  • Humans
  • Iron / metabolism
  • Lactoperoxidase / metabolism*
  • Molecular Sequence Data
  • Peroxidase / genetics
  • Peroxidase / isolation & purification
  • Peroxidase / metabolism*
  • Peroxidases / metabolism*
  • Rats
  • Sequence Homology, Nucleic Acid
  • Spectrophotometry, Atomic
  • Structure-Activity Relationship

Substances

  • Calcium-Binding Proteins
  • Iron
  • Lactoperoxidase
  • Peroxidases
  • Peroxidase
  • Calcium