Molecular flow quantified beyond the diffraction limit by spatiotemporal image correlation of structured illumination microscopy data

Biophys J. 2014 Nov 4;107(9):L21-3. doi: 10.1016/j.bpj.2014.09.018.

Abstract

We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps. The flow was found to be retrograde and radially directed throughout the periphery of T-cells during synapse formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • CD4 Antigens / metabolism
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Jurkat Cells
  • Microscopy, Fluorescence / methods*
  • Molecular Imaging / methods
  • Spectrum Analysis / methods
  • T-Lymphocytes / metabolism

Substances

  • Actins
  • CD4 Antigens