Upregulation of CD11A on hematopoietic stem cells denotes the loss of long-term reconstitution potential

Stem Cell Reports. 2014 Nov 11;3(5):707-15. doi: 10.1016/j.stemcr.2014.09.007. Epub 2014 Oct 16.


Small numbers of hematopoietic stem cells (HSCs) generate large numbers of mature effector cells through the successive amplification of transiently proliferating progenitor cells. HSCs and their downstream progenitors have been extensively characterized based on their cell-surface phenotype and functional activities during transplantation assays. These cells dynamically lose and acquire specific sets of surface markers during differentiation, leading to the identification of markers that allow for more refined separation of HSCs from early hematopoietic progenitors. Here, we describe a marker, CD11A, which allows for the enhanced purification of mouse HSCs. We show through in vivo transplantations that upregulation of CD11A on HSCs denotes the loss of their long-term reconstitution potential. Surprisingly, nearly half of phenotypic HSCs (defined as Lin-KIT(+)SCA-1(+)CD150(+)CD34-) are CD11A(+) and lack long-term self-renewal potential. We propose that CD11A(+)Lin-KIT(+)SCA-1(+)CD150(+)CD34- cells are multipotent progenitors and CD11A-Lin-KIT(+)SCA-1(+)CD150(+)CD34- cells are true HSCs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / metabolism
  • Antigens, CD34 / metabolism
  • Antigens, Ly / metabolism
  • CD11a Antigen / genetics
  • CD11a Antigen / metabolism*
  • Cell Differentiation*
  • Cell Proliferation*
  • Flow Cytometry
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / metabolism*
  • Membrane Proteins / metabolism
  • Mice, Inbred C57BL
  • Proto-Oncogene Proteins c-kit / metabolism
  • Receptors, Cell Surface / metabolism
  • Signaling Lymphocytic Activation Molecule Family Member 1
  • Up-Regulation*


  • Antigens, CD
  • Antigens, CD34
  • Antigens, Ly
  • CD11a Antigen
  • Ly6a protein, mouse
  • Membrane Proteins
  • Receptors, Cell Surface
  • Signaling Lymphocytic Activation Molecule Family Member 1
  • Proto-Oncogene Proteins c-kit

Associated data

  • GEO/GSE34723
  • GEO/GSM148041
  • GEO/GSM1483039
  • GEO/GSM1483040
  • GEO/GSM1483042
  • GEO/GSM1483043