Characterization of a purF operon mutation which affects colicin V production

J Bacteriol. 1989 Jun;171(6):3158-61. doi: 10.1128/jb.171.6.3158-3161.1989.


A mini-Tn10-kan insertion mutation identified a gene in the chromosome of Escherichia coli required for colicin V production from plasmid pColV-K30. With the complete restriction map of E. coli, the mutation was rapidly mapped to 50.0 min, within the purF operon. Sequence analysis showed that the insertion occurred in a gene with no previously known function which is located directly upstream of purF. We designated this gene cvpA for colicin V production. The mutant requires adenine for growth, probably because of a polar effect on purF expression. However, an adenine auxotroph showed no defect in colicin V production, suggesting that the cvpA mutation is responsible for the effect on colicin V production. Two possible models of cvpA1 allele function are discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Bacteriocin Plasmids*
  • Chromosomes, Bacterial / physiology
  • Cloning, Molecular
  • Colicins / biosynthesis*
  • DNA Transposable Elements
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics*
  • Gene Expression Regulation
  • Genes, Bacterial*
  • Operon
  • Plasmids*
  • Purines / physiology
  • Restriction Mapping


  • Colicins
  • DNA Transposable Elements
  • DNA, Bacterial
  • Purines