A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus

J Virol Methods. 1989 Feb;23(2):157-67. doi: 10.1016/0166-0934(89)90129-8.

Abstract

We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within 16 h following virus inoculation. In addition, both assays resulted in considerable savings of reagents because the assays were carried out in 96-well microtiter plates. These assays should prove useful in the preparation and study of neutralizing antibodies directed against human cytomegalovirus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antibodies, Viral / analysis*
  • Antigens, Viral / immunology
  • Cells, Cultured
  • Cytomegalovirus / immunology*
  • Fibroblasts
  • Humans
  • Immediate-Early Proteins*
  • Immunoblotting
  • Neutralization Tests
  • Nuclear Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral
  • Immediate-Early Proteins
  • Nuclear Proteins
  • immediate-early proteins, cytomegalovirus