Cytochemical localization of plasma membrane enzyme markers during interiorization of tachyzoites of Toxoplasma gondii by macrophages

J Protozool. Mar-Apr 1989;36(2):164-70. doi: 10.1111/j.1550-7408.1989.tb01066.x.

Abstract

The enzyme activity of Mg++-ATPase, Na+-K+-ATPase, 5'-nucleotidase and NAD(P)H-oxidase was cytochemically detected at the ultrastructural level in mouse peritoneal macrophages infected with untreated and with specific antibody-coated Toxoplasma gondii tachyzoites. The Mg++-ATPase and 5'-nucleotidase were distributed throughout the macrophages' plasma membrane but were not observed in the membrane lining endocytic vacuoles containing ingested parasites; however, Na+-K+-ATPase activity was detected in the macrophages' plasma membrane as well as in the parasitophorous vacuoles that contained untreated or specific antibody-coated parasites. Reaction product, indicative of NAD(P)H-oxidase, was detected in the parasitophorous vacuoles that contained only specific antibody-coated parasites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5'-Nucleotidase
  • Adenosine Triphosphatases / analysis*
  • Animals
  • Ca(2+) Mg(2+)-ATPase / analysis
  • Cell Membrane / enzymology
  • Histocytochemistry
  • Macrophages / enzymology
  • Macrophages / parasitology*
  • Macrophages / ultrastructure
  • Microscopy, Electron
  • NADH, NADPH Oxidoreductases / analysis*
  • NADPH Oxidases
  • Nucleotidases / analysis*
  • Sodium-Potassium-Exchanging ATPase / analysis
  • Toxoplasma / physiology*
  • Toxoplasma / ultrastructure

Substances

  • NADH, NADPH Oxidoreductases
  • NADPH Oxidases
  • Nucleotidases
  • 5'-Nucleotidase
  • Adenosine Triphosphatases
  • Ca(2+) Mg(2+)-ATPase
  • Sodium-Potassium-Exchanging ATPase