T-cell-mediated clearance of mouse hepatitis virus strain JHM from the central nervous system

J Virol. 1989 Jul;63(7):3051-6. doi: 10.1128/JVI.63.7.3051-3056.1989.

Abstract

Clearance of the neurotropic JHM strain of mouse hepatitis virus from the central nervous system was examined by the transfer of spleen cells from immunized donors. A T cell with the surface phenotype of Thy1.2+ CD4+ CD8- asialo-GM1+ Mac-1- was found to be necessary for viral clearance. The surface phenotype and adherence to nylon wool suggest that these cells are activated helper-inducer T cells. Adoptive transfer to congenic histocompatibility strains demonstrated the necessity for compatibility at the D locus of the major histocompatibility complex. The expression of the CD4 surface marker and the requirement for major histocompatibility complex class I were further studied by the transfer of cells to recipients treated with anti-CD4 or anti-CD8 monoclonal antibodies. Treatment of recipients with either the anti-CD8 or the anti-CD4 antibodies inhibited virus clearance from the central nervous system. This suggests that the CD4+ cell acts as a helper and that virus is cleared from the central nervous system. This suggests that the CD4+ cell acts as a helper and that virus is cleared from the central nervous system by CD8+ cells that recognize viral antigen in the context of the H-2Db gene product.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / therapeutic use
  • Antigens, Surface / analysis
  • Antigens, Viral / immunology
  • Cell Line
  • Central Nervous System / immunology
  • Central Nervous System / microbiology*
  • Hepatitis, Viral, Animal / immunology*
  • Hypersensitivity, Delayed
  • Immunity, Cellular
  • Immunization, Passive
  • Mice
  • Mice, Inbred Strains
  • Murine hepatitis virus / immunology*
  • Phenotype
  • Species Specificity
  • T-Lymphocytes / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Antigens, Viral