The spliced BZLF1 gene of Epstein-Barr virus (EBV) transactivates an early EBV promoter and induces the virus productive cycle

J Virol. 1989 Jul;63(7):3109-16. doi: 10.1128/JVI.63.7.3109-3116.1989.


A spliced cDNA spanning the Epstein-Barr virus BZLF1 gene expresses the BZLF1 protein and is active in inducing the virus productive cycle. A deletion mutant which lacks the N-terminal half of the protein is inactive. Cotransfection experiments in EBV-negative B-lymphocyte cell lines demonstrated that the BZLF1 gene activates the promoter for the BSLF2 + BMLF1 gene in the absence of any other EBV gene product. These results confirmed that the spliced BZLF1 gene is the transactivating gene structure in BamHI-Z.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • DNA, Recombinant
  • Gene Expression Regulation*
  • Genes*
  • Genes, Viral*
  • Herpesvirus 4, Human / genetics*
  • Herpesvirus 4, Human / physiology
  • Humans
  • Molecular Sequence Data
  • Oncogene Proteins, Viral / genetics*
  • Plasmids
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Transcription, Genetic
  • Transfection
  • Virus Replication*


  • DNA, Recombinant
  • Oncogene Proteins, Viral
  • RNA, Messenger