Effect of dietary astaxanthin on free radical scavenging capacity and nitrite stress tolerance of postlarvae shrimp, Pleoticus muelleri

J Agric Food Chem. 2014 Dec 24;62(51):12326-31. doi: 10.1021/jf503754q. Epub 2014 Dec 10.


The aim of this study was to investigate the effect of astaxanthin feed supplementation and environmental nitrite stress in postlarvae of Pleoticus muelleri (15 ± 0.004 mg initial weight) under culture conditions. Diets containing three levels of astaxanthin, 0 mg kg(-1) of diet (C0), 100 mg kg(-1) of diet (C(100)), and 300 mg kg(-1) of diet (C(300)), were used. Postlarvae fed with each diet were exposed to different concentrations of nitrite (NO(2)Na) (0-200 mg L(-1)). The 96 h median lethal concentration (LC50) values of nitrite N were 76.3, 89.7, and 157 mg L(-1) for shrimps fed to C0, C(100), and C(300). The scavenging properties were evaluated against the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by electron resonance spectroscopy (EPR). For all feed treatments, the extracts exhibited strong DPPH scavenging activity; however, shrimp fed with C(100) and C(300) showed the greatest activity to quench DPPH (62 and 59%, respectively) with respect to C0 (43%). It can be concluded that astaxanthin acts as a protector of nitrite stress in P. muelleri.

Keywords: Pleoticus muelleri; astaxanthin; histopathology; nitrite; scavenging capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed / analysis*
  • Animals
  • Decapoda / chemistry
  • Decapoda / growth & development
  • Decapoda / physiology*
  • Dietary Supplements / analysis*
  • Free Radical Scavengers / analysis
  • Free Radical Scavengers / metabolism*
  • Free Radicals / metabolism
  • Larva / growth & development
  • Larva / metabolism
  • Nitrites / metabolism*
  • Stress, Physiological
  • Xanthophylls / analysis
  • Xanthophylls / metabolism


  • Free Radical Scavengers
  • Free Radicals
  • Nitrites
  • Xanthophylls
  • astaxanthine