SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism

Oncogene. 1989 May;4(5):601-8.


SV40 and polyoma virus induce a mitotic host reaction in confluent, Go-arrested primary mouse kidney cell cultures. To define the primary effects of infection we constructed a cDNA library corresponding to polyA+ mRNA isolated shortly after onset of polyoma T-antigen synthesis. By differential screening of the library we have isolated and then sequenced cDNA recombinant 24p3; determined by Northern blotting, 24p3 mRNA steady state levels increased in parallel with polyoma and SV40 T-antigen synthesis. Since this rapid and early increase was particularly striking (14-20 fold) in SV40-infected cells, we studied the molecular mechanism of induction in this virus-cell system. We show that wt SV40 large T-antigen is required for the increase in 24p3 mRNA levels. The results tend to exclude that this increase is due to an SV40-induced stabilization of the 24p3 mRNA, or to an SV40-induced stimulation of transcription of the 24p3 gene; they are compatible with the working hypothesis that SV40 large T-antigen increases the efficiency of processing, possibly splicing, of the 24p3 pre-mRNA. The biological implications of these results are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Polyomavirus Transforming / biosynthesis
  • Base Sequence
  • Blotting, Northern
  • Cells, Cultured
  • Gene Expression Regulation*
  • Mice
  • Molecular Sequence Data
  • Polyomavirus / physiology
  • RNA Processing, Post-Transcriptional*
  • RNA, Messenger / metabolism*
  • Simian virus 40 / genetics
  • Simian virus 40 / physiology*
  • Transcription, Genetic


  • Antigens, Polyomavirus Transforming
  • RNA, Messenger

Associated data

  • GENBANK/X14607