Rerouting of plant late endocytic trafficking toward a pathogen interface

Traffic. 2015 Feb;16(2):204-26. doi: 10.1111/tra.12245. Epub 2015 Jan 4.

Abstract

A number of plant pathogenic and symbiotic microbes produce specialized cellular structures that invade host cells where they remain enveloped by host-derived membranes. The mechanisms underlying the biogenesis and functions of host-microbe interfaces are poorly understood. Here, we show that plant late endocytic trafficking is diverted toward the extrahaustorial membrane (EHM); a host-pathogen interface that develops in plant cells invaded by Irish potato famine pathogen Phytophthora infestans. A late endosome and tonoplast marker protein Rab7 GTPase RabG3c, but not a tonoplast-localized sucrose transporter, is recruited to the EHM, suggesting specific rerouting of vacuole-targeted late endosomes to a host-pathogen interface. We revealed the dynamic nature of this process by showing that, upon activation, a cell surface immune receptor traffics toward the haustorial interface. Our work provides insight into the biogenesis of the EHM and reveals dynamic processes that recruit membrane compartments and immune receptors to this host-pathogen interface.

Keywords: AVRblb2; Phytophthora infestans; RabG3c; effector; extrahaustorial membrane; haustorium; late endosome.

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Endocytosis*
  • Endosomes / metabolism*
  • Host-Pathogen Interactions*
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism
  • Phytophthora infestans / pathogenicity
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Protein Transport
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • Tobacco / genetics
  • Tobacco / metabolism*
  • Tobacco / microbiology
  • rab GTP-Binding Proteins / genetics
  • rab GTP-Binding Proteins / metabolism

Substances

  • Arabidopsis Proteins
  • Membrane Transport Proteins
  • Plant Proteins
  • sucrose transport protein, plant
  • Protein Kinases
  • BAK1 protein, Arabidopsis
  • FLS2 protein, Arabidopsis
  • Protein-Serine-Threonine Kinases
  • rab GTP-Binding Proteins