High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

Mol Biosyst. 2015 Feb;11(2):361-5. doi: 10.1039/c4mb00556b. Epub 2014 Nov 28.

Abstract

Using a wheat germ cell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell-Free System
  • Isotope Labeling / methods*
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Protein Biosynthesis*
  • Proteomics / methods*
  • Receptors, Neurotransmitter / metabolism
  • Triticum / chemistry*

Substances

  • Membrane Proteins
  • Receptors, Neurotransmitter