Abstract
The activity and dynamic nature of TATA-binding protein (TBP) crucial to RNA polymerase II-mediated transcription is under control of the Mot1p and NC2 complexes. Here we show that both TBP regulatory factors play 'hidden' roles in ensuring transcription fidelity by restricting anti-sense non-coding RNA (ncRNA) synthesis. Production of anti-sense ncRNA transcripts is suppressed by Mot1p- and NC2-mediated release of TBP from binding sites at the 3'-end of genes. In this, Mot1p and NC2 collaborate with the Nrd1p-Nab3p-Sen1p (NNS) complex that terminates the synthesis of anti-sense ncRNAs. In several cases anti-sense ncRNA expression interferes with expression of the cognate sense transcript. Our data reveal a novel regulatory mechanism to suppress anti-sense ncRNA expression and pre-initiation complex (PIC) formation at spurious sites.
© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3' Flanking Region
-
Adenosine Triphosphatases / metabolism*
-
Chromatin / metabolism
-
Gene Expression Regulation, Fungal*
-
Phosphoproteins / metabolism*
-
Promoter Regions, Genetic
-
RNA, Antisense / biosynthesis*
-
RNA, Untranslated / biosynthesis
-
RNA-Binding Proteins / genetics
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / growth & development
-
Saccharomyces cerevisiae / metabolism
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism*
-
TATA-Binding Protein Associated Factors / metabolism*
-
TATA-Box Binding Protein / metabolism*
-
Transcription Factors / metabolism*
-
Transcription, Genetic*
Substances
-
Chromatin
-
NRD1 protein, S cerevisiae
-
Phosphoproteins
-
RNA, Antisense
-
RNA, Untranslated
-
RNA-Binding Proteins
-
SPT15 protein, S cerevisiae
-
Saccharomyces cerevisiae Proteins
-
TATA-Binding Protein Associated Factors
-
TATA-Box Binding Protein
-
Transcription Factors
-
down-regulator of transcription 1
-
Adenosine Triphosphatases
-
MOT1 protein, S cerevisiae