Effect of ACTH on the stability of mRNAs encoding bovine adrenocortical P-450scc, P-45011 beta, P-45017 alpha, P-450C21 and adrenodoxin

Biochem Biophys Res Commun. 1989 May 15;160(3):1227-32. doi: 10.1016/s0006-291x(89)80134-2.


ACTH treatment of bovine adrenocortical cells in primary culture causes increased accumulation of mRNAs encoding cytochromes P-450SCC, P-450(11)beta, P-450(17) alpha, P-450C21 and adrenodoxin as well as increased transcriptional activity of their respective genes. In this study we have shown that ACTH does not greatly affect the half-life of mRNAs encoding P-450(11)beta, P-450(17)alpha, P-450C21 and adrenodoxin. However, in the case of P-450SCC mRNA, ACTH causes a five-fold increase in the half-life leading to a significant stabilization of P-450SCC mRNA. Thus it appears that the levels of mRNAs encoding P-450(11)beta, P-450(17)alpha, P-450C21 and adrenodoxin are regulated by ACTH primarily at the transcriptional level, while that for P-450SCC is regulated at both the transcriptional and post-transcriptional levels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenal Cortex / drug effects
  • Adrenal Cortex / enzymology*
  • Adrenocorticotropic Hormone / pharmacology*
  • Adrenodoxin / genetics*
  • Animals
  • Cattle
  • Cells, Cultured
  • Cholesterol Side-Chain Cleavage Enzyme / genetics
  • Cytochrome P-450 Enzyme System / genetics*
  • Drug Stability
  • Gene Expression Regulation / drug effects*
  • Half-Life
  • Protein Biosynthesis / drug effects
  • RNA, Messenger / metabolism*
  • Transcription, Genetic / drug effects


  • RNA, Messenger
  • Adrenodoxin
  • Adrenocorticotropic Hormone
  • Cytochrome P-450 Enzyme System
  • Cholesterol Side-Chain Cleavage Enzyme