Synthesis and characterization of pectin derivative with antitumor property against Caco-2 colon cancer cells

Carbohydr Polym. 2015 Jan 22;115:139-45. doi: 10.1016/j.carbpol.2014.08.085. Epub 2014 Sep 2.


New pectin derivative (Pec-MA) was obtained in specific reaction conditions. The presence of maleoyl groups in Pec-MA structure was confirmed by (1)H NMR and FTIR spectroscopy. The substitution degree of Pec-MA (DS=24%) was determined by (1)H NMR. The properties of Pec-MA were investigated through WAXS, TGA/DTG, SEM and zeta potential techniques. The Pec-MA presented amorphous characteristics and higher-thermal stability compared to raw pectin (Pec). In addition, considerable morphological differences between Pec-MA and Pec were observed by SEM. The cytotoxic effect on the Caco-2 cells showed that the Pec-MA significantly inhibited the growth of colon cancer cells whereas the Pec-MA does not show any cytotoxic effect on the VERO healthy cells. This result opens new perspectives for the manufacture of biomaterials based on Pec with anti-tumor properties.

Keywords: Antitumor property; Biopolymers; Caco-2 cells; Pectin; Pectin derivative; Vero cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Caco-2 Cells
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chlorocebus aethiops
  • Colonic Neoplasms / drug therapy
  • Colonic Neoplasms / pathology*
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Humans
  • Molecular Structure
  • Pectins / chemical synthesis
  • Pectins / chemistry
  • Pectins / pharmacology*
  • Structure-Activity Relationship
  • Vero Cells


  • Antineoplastic Agents
  • Pectins