Association of arteriosclerosis index and oxidative stress markers in school children

Pediatr Int. 2015 Jun;57(3):449-54. doi: 10.1111/ped.12545. Epub 2015 Feb 7.


Background: Recent years have seen increased numbers of children with conditions that contribute strongly to atherosclerotic disease, such as passive smoking, obesity, and dyslipidemia. In the present study, we evaluated the utility of non-invasive urinary markers in preventing lifestyle-related diseases by comparing lipid metabolism-related parameters with oxidative stress markers in school children.

Methods: Subjects were 85 first-grade students. The variables examined included the smoking in subjects' household; exercise habits; height and weight; blood pressure; and plasma total cholesterol, high-density lipoprotein cholesterol, triglyceride, leptin, blood sugar, urinary cotinine, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and 8-isoprostaglandin F2α (IsoP).

Results: Of the subjects, 10.6% were obese (% overweight ≥ 20%), 3.5% had a high-risk arteriosclerosis index (AI; 3 ≤ AI < 5), and 29.4% were passive smokers. No significant differences were seen between boys and girls for any of the measurement parameters. Both urinary 8-OHdG (6.8-24.5 ng/mg creatinine) and IsoP (0.9-7.4 ng/mg creatinine) were detected in all subjects, and a significant positive correlation was seen between the two markers. On multiple regression analysis using AI as an objective variable and all non-invasive markers as explanatory variables, urinary IsoP correlated most strongly with AI (P ≤ 0.01).

Conclusions: Risk factors for atherosclerosis in adults, such as obesity and hypercholesterolemia, are associated with oxidative stress and inflammation. The present findings of the strongest correlation between urinary IsoP and AI suggest that urinary IsoP may serve as a non-invasive and effective early marker in predicting risk in children of developing lifestyle-related diseases.

Keywords: 8-hydroxy-2′-deoxyguanosine; 8-isoprostaglandin F2α; arteriosclerosis index; oxidative stress marker; school children.

MeSH terms

  • Arteriosclerosis / epidemiology
  • Arteriosclerosis / etiology
  • Arteriosclerosis / metabolism*
  • Biomarkers / metabolism*
  • Child
  • Dinoprost / analogs & derivatives
  • Dinoprost / urine
  • Dyslipidemias / complications*
  • Dyslipidemias / epidemiology
  • Dyslipidemias / metabolism
  • Female
  • Humans
  • Incidence
  • Japan / epidemiology
  • Lipids / blood
  • Male
  • Overweight / complications*
  • Overweight / metabolism
  • Oxidative Stress*
  • Risk Assessment / methods*
  • Risk Factors
  • Schools


  • Biomarkers
  • Lipids
  • 8-epi-prostaglandin F2alpha
  • Dinoprost