Thyroid epithelial cell transformation by a retroviral vector expressing SV40 large T

Br J Cancer. 1989 May;59(5):755-60. doi: 10.1038/bjc.1989.158.


A recombinant murine retroviral vector encoding the SV40 virus large T antigen was used to infect stably an immortal line of differentiated rat thyroid epithelial cells, FRTL-5. Expression of SV40 T transformed these cells to anchorage independence and tumorigenicity but did not alter morphology or abolish tissue-specific functions and growth factor requirements. The resulting phenotype provides a model of well-differentiated human thyroid cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Polyomavirus Transforming / genetics*
  • Cell Line
  • Cell Transformation, Neoplastic*
  • Epithelium / pathology
  • Genetic Vectors*
  • Plasmids
  • Rats
  • Simian virus 40 / immunology
  • Thyroid Gland / pathology*
  • Thyroid Neoplasms / etiology
  • Thyroid Neoplasms / genetics*


  • Antigens, Polyomavirus Transforming