Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control

Cell. 1989 Jun 16;57(6):1009-16. doi: 10.1016/0092-8674(89)90339-5.

Abstract

Fission yeast cdc25+ and wee1+ interact genetically with cdc2+ in the regulation of cell division, respectively as a mitotic activator and inhibitor. cdc25+ is normally essential for mitosis, but this requirement is alleviated in a loss-of-function wee1 mutant background. A plasmid-borne sequence, other than wee1+, that causes a cdc25ts wee1- double mutant to revert to a temperature-sensitive cdc phenotype has been isolated. The gene carried by this plasmid is called bws1+ (for bypass of wee suppression). bws1+ also bypasses the ability of alleles of cdc2 that confer a wee phenotype (cdc2w) to suppress loss-of-function cdc25 mutants. The nucleotide sequence of bws1+ shows that the predicted protein shares 81% amino acid identity with the catalytic subunit of mammalian type 1 protein phosphatase. Thus a genetic screen that might have yielded a protein kinase (wee1+) uncovered a phosphatase that also appears to be involved in the pathway of mitotic control.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Endonucleases / pharmacology
  • Fungal Proteins / genetics
  • Genes, Fungal*
  • Genes, Lethal
  • Mitosis*
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Phosphoprotein Phosphatases / genetics*
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / genetics*
  • Single-Strand Specific DNA and RNA Endonucleases
  • Transcription, Genetic

Substances

  • Fungal Proteins
  • Endonucleases
  • Phosphoprotein Phosphatases
  • Single-Strand Specific DNA and RNA Endonucleases

Associated data

  • GENBANK/M27075