The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases

Cell. 1989 Jun 16;57(6):997-1007. doi: 10.1016/0092-8674(89)90338-3.


S. pombe dis mutants block mitotic chromosome disjunction in a manner reminiscent of aneuploidy formation, and belong to three distinct genes, dis1-dis3. We cloned two independent genomic DNAs that complemented both the cold-sensitive and caffeine-hypersensitive phenotype of dis2-11. These genes, dis2+ and a suppressor sds21+, encode proteins (calculated MW 37,000) with similar predicted amino acid sequences. dis2+ and sds21+ have overlapping functions, and disruptants are lethal only when both genes are disrupted. The gene products identified by anti-dis2 serum are enriched in nuclei. By hybridization, we obtained two cDNA clones from mouse and one genomic clone from S. cerevisiae; the latter complements S. pombe dis2-11. These dis2+ and similar polypeptides of yeasts and mouse are found to be highly homologous (75%-90% identical) to rabbit protein phosphatase 1. The implications of these findings are discussed with regard to mitotic control.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caffeine / pharmacology
  • Chromosome Mapping
  • Cloning, Molecular
  • Cold Temperature
  • DNA, Fungal / genetics
  • Fluorescent Antibody Technique
  • Fungal Proteins / genetics
  • Genes, Fungal*
  • Genes, Lethal
  • Genetic Complementation Test
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Nondisjunction, Genetic*
  • Phosphoprotein Phosphatases / genetics*
  • Phosphoprotein Phosphatases / immunology
  • Protein Phosphatase 1
  • Saccharomyces cerevisiae / genetics
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / genetics*


  • DNA, Fungal
  • Fungal Proteins
  • Caffeine
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1

Associated data

  • GENBANK/M27068
  • GENBANK/M27069
  • GENBANK/M27070
  • GENBANK/M27071
  • GENBANK/M27073