Motility and fertility of cryopreserved semen in Persian sturgeon, Acipenser persicus, stored for 30-60 min after thawing

Cryobiology. 2014 Dec;69(3):500-2. doi: 10.1016/j.cryobiol.2014.10.006. Epub 2014 Oct 18.

Abstract

We investigated the effect of storage times of frozen-thawed Persian sturgeon (Acipenser persicus) semen on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30, and 60 min at 4°C post-thawing. Frozen thawed semen analyzed immediately after thawing had similar quality characteristics as fresh semen. For cryopreserved semen stored for 30 min after thawing the characteristics did not differ to fresh semen and cryopreserved semen. For cryopreserved semen stored for 60 min a significant decline in the parameters was observed. Fertilization and hatching rates were not affected by storage times of maximally 30 min of storage.

Keywords: Cryopreservation; Fertilization; Persian sturgeon; Sperm motility; Sperm storage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cryopreservation / methods
  • Cryopreservation / veterinary*
  • Female
  • Fertilization
  • Fishes / physiology*
  • Male
  • Semen / cytology*
  • Semen Preservation / methods
  • Semen Preservation / veterinary*
  • Sperm Motility
  • Spermatozoa / cytology