RILP interacts with HOPS complex via VPS41 subunit to regulate endocytic trafficking

Sci Rep. 2014 Dec 2:4:7282. doi: 10.1038/srep07282.

Abstract

The HOPS complex serves as a tethering complex with GEF activity for Ypt7p in yeast to regulate late endosomal membrane maturation. While the role of HOPS complex is well established in yeast cells, its functional and mechanistic aspects in mammalian cells are less well defined. In this study, we report that RILP, a downstream effector of Rab7, interacts with HOPS complex and recruits HOPS subunits to the late endosomal compartment. Structurally, the amino-terminal portion of RILP interacts with HOPS complex. Unexpectedly, this interaction is independent of Rab7. VPS41 subunit of HOPS complex was defined to be the major partner for interacting with RILP. The carboxyl-terminal region of VPS41 was mapped to be responsible for the interaction. Functionally, either depletion of VPS41 by shRNA or overexpression of VPS41 C-terminal half retarded EGF-induced degradation of EGFR. These results suggest that interaction of RILP with HOPS complex via VPS41 plays a role in endocytic trafficking of EGFR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / metabolism*
  • Cell Line, Tumor
  • Epidermal Growth Factor / metabolism
  • ErbB Receptors
  • HeLa Cells
  • Humans
  • MCF-7 Cells
  • Nuclear Proteins / metabolism*
  • Protein Transport / physiology*
  • Transport Vesicles / metabolism*
  • Transport Vesicles / physiology*
  • Vesicular Transport Proteins / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Nuclear Proteins
  • RILP protein, human
  • VPS41 protein, human
  • Vesicular Transport Proteins
  • Epidermal Growth Factor
  • EGFR protein, human
  • ErbB Receptors