Opposite effects of GPR120 and GPR40 on cell motile activity induced by ethionine in liver epithelial cells

Shuhei Ishii; Miku Hirane; Sayumi Kato; Nobuyuki Fukushima; Toshifumi Tsujiuchi

doi:10.1016/j.bbrc.2014.11.047

Opposite effects of GPR120 and GPR40 on cell motile activity induced by ethionine in liver epithelial cells

Biochem Biophys Res Commun. 2015 Jan 2;456(1):135-8. doi: 10.1016/j.bbrc.2014.11.047. Epub 2014 Nov 21.

Authors

Shuhei Ishii¹, Miku Hirane¹, Sayumi Kato¹, Nobuyuki Fukushima², Toshifumi Tsujiuchi³

Affiliations

¹ Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan.
² Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan.
³ Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan. Electronic address: ttujiuch@life.kindai.ac.jp.

PMID: 25446111
DOI: 10.1016/j.bbrc.2014.11.047

Abstract

Free fatty acids (FFAs) are dietary nutrients which act as ligands for FFAs receptors. G-protein-coupled receptor 120 (GPR120) and GPR40 are activated by long and medium chain FFAs. In the present study, we investigated the role of the GPR120 and GPR40 in cell motile activity stimulated by ethionine in rat liver epithelial WB-F344 cells. Cells were treated with ethionine at a concentration of 10μM every 24h for 2days. The expression levels of the Gpr120 and Gpr40 genes in WB-F344 cells treated ethionine were significantly higher than those in untreated cells. In cell motility assay, the cell motile activity of WB-F344 cells was markedly elevated by ethionine, compared with untreated cells. To evaluate the effects of GPR120 on the cell motile activity by ethionine, we established GPR120 knockdown cells from WB-F344 cells. The cell motile activity stimulated by ethionine was significantly suppressed by GPR120 knockdown. In addition, a potent GPR40 antagonist GW1100 enhanced the cell motile activity by ethionine. These results suggest that opposite effects of GPR120 and GPR40 may be involved in the cell motile activity stimulated by ethionine in WB-F344 cells.

Keywords: Cell motility; Ethionine; GPR120; GPR40; Liver cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Movement
Cell Proliferation
Epithelial Cells / metabolism*
Ethionine / chemistry*
Liver / metabolism*
Male
Polymerase Chain Reaction
Rats
Rats, Inbred F344
Receptors, G-Protein-Coupled / metabolism*

Substances

Ffar4 protein, rat
G-protein-coupled receptor 40, rat
Receptors, G-Protein-Coupled
Ethionine