CD3-negative lymphoproliferative disease of granular lymphocytes containing Epstein-Barr viral DNA

J Clin Invest. 1989 Jul;84(1):51-5. doi: 10.1172/JCI114168.


Lymphoproliferative disease of granular lymphocytes (LDGL) is a heterogeneous disorder and the pathogenesis is likely to be complex. Some patients with chronic active EBV (CAEBV) infection also have LDGL. To investigate the relationship between EBV infection and the pathogenesis of LDGL, we conducted a survey for EBV DNA sequences by Southern blot analysis of DNA obtained from the peripheral blood of seven patients with LDGL, including one with CAEBV infection. Interestingly, EBV DNA was detected in the sample from the patient with CAEBV infection, and in the samples from four other patients with CD3-LDGL. Moreover, a single band for the joined termini of the EBV genome was demonstrated in two samples, suggesting a clonal disorder of those LDGL. These findings strongly suggest that EBV may play a pathogenic role in some cases of LDGL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antibodies, Viral / immunology
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • Antigens, Viral / immunology
  • Blotting, Southern
  • CD3 Complex
  • Capsid / immunology
  • Child
  • Chronic Disease
  • DNA, Viral / analysis*
  • DNA, Viral / genetics
  • Female
  • Granulocytes / immunology*
  • Granulocytes / microbiology
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Lymphoproliferative Disorders / immunology*
  • Lymphoproliferative Disorders / microbiology
  • Male
  • Middle Aged
  • Nucleic Acid Hybridization
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology*


  • Antibodies, Viral
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Viral
  • CD3 Complex
  • DNA, Viral
  • Receptors, Antigen, T-Cell