IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment

Dev Cell. 2014 Nov 10;31(3):279-290. doi: 10.1016/j.devcel.2014.09.011. Epub 2014 Oct 30.


Vertebrate hedgehog signaling is coordinated by the differential localization of the receptors patched-1 and Smoothened in the primary cilium. Cilia assembly is mediated by intraflagellar transport (IFT), and cilia defects disrupt hedgehog signaling, causing many structural birth defects. We generated Ift25 and Ift27 knockout mice and show that they have structural birth defects indicative of hedgehog signaling dysfunction. Surprisingly, ciliary assembly is not affected, but abnormal hedgehog signaling is observed in conjunction with ciliary accumulation of patched-1 and Smoothened. Similarly, Smoothened accumulates in cilia on cells mutated for BBSome components or the BBS binding protein/regulator Lztfl1. Interestingly, the BBSome and Lztfl1 accumulate to high levels in Ift27 mutant cilia. Because Lztfl1 mutant cells accumulate BBSome but not IFT27, it is likely that Lztfl1 functions downstream of IFT27 to couple the BBSome to the IFT particle for coordinated removal of patched-1 and Smoothened from cilia during hedgehog signaling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Cilia / metabolism*
  • Flagella / metabolism
  • Hedgehog Proteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Receptors, Cell Surface / metabolism
  • Signal Transduction*
  • Transcription Factors / metabolism
  • rab GTP-Binding Proteins / metabolism*


  • Hedgehog Proteins
  • Lztfl1 protein, mouse
  • Receptors, Cell Surface
  • Transcription Factors
  • intraflagellar transport 27 protein, mouse
  • rab GTP-Binding Proteins