Structure and expression of an integrated human papillomavirus type 16 genome amplified in a cervical carcinoma cell line

H Shirasawa; Y Tomita; A Fuse; T Yamamoto; H Tanzawa; S Sekiya; H Takamizawa; B Simizu

doi:10.1099/0022-1317-70-7-1913

Structure and expression of an integrated human papillomavirus type 16 genome amplified in a cervical carcinoma cell line

J Gen Virol. 1989 Jul:70 ( Pt 7):1913-9. doi: 10.1099/0022-1317-70-7-1913.

Authors

H Shirasawa¹, Y Tomita, A Fuse, T Yamamoto, H Tanzawa, S Sekiya, H Takamizawa, B Simizu

Affiliation

¹ Department of Microbiology, School of Medicine, Chiba University, Japan.

PMID: 2544671
DOI: 10.1099/0022-1317-70-7-1913

Abstract

A cellular sequence containing the integrated human papillomavirus type 16 genome in a cervical carcinoma cell line QG-U was cloned and analysed. The transcriptionally active viral genome disrupted at the E2 and L2 open reading frames was amplified with its flanking sequences.

MeSH terms

Base Sequence
Carcinoma / genetics
Carcinoma / microbiology*
Cell Line
DNA Probes, HPV
DNA, Viral / isolation & purification
Female
Gene Amplification*
Genes, Viral*
Humans
Molecular Sequence Data
Papillomaviridae / genetics*
Restriction Mapping
Transcription, Genetic
Uterine Cervical Neoplasms / genetics
Uterine Cervical Neoplasms / microbiology*

Substances

DNA Probes, HPV
DNA, Viral

Associated data

GENBANK/D00486
GENBANK/D00487