Electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary

Zhenqing Li; Ronglin Yang; Qi Wang; Dawei Zhang; Songlin Zhuang; Yoshinori Yamaguchi

doi:10.1016/j.ab.2014.11.002

Electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary

Anal Biochem. 2015 Feb 15:471:70-2. doi: 10.1016/j.ab.2014.11.002. Epub 2014 Nov 13.

Authors

Zhenqing Li¹, Ronglin Yang², Qi Wang², Dawei Zhang², Songlin Zhuang², Yoshinori Yamaguchi³

Affiliations

¹ Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address: zhenqingli@163.com.
² Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.
³ Photonics and Biomedical Research Institute, Department of Physics, Faculty of Science, East China University of Science and Technology, Shanghai 200237, China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita City, Osaka 565-0871, Japan. Electronic address: yoshi.yamaguchi@ap.eng.osaka-u.ac.jp.

PMID: 25447490
DOI: 10.1016/j.ab.2014.11.002

Abstract

Periodontitis is a prevalent inflammatory disease caused by different species of anaerobic bacteria such as Porphyromonas gingivalis (P.g), Treponema denticola (T.d), and Tannerella forsythia (T.f). We compared the separation result of DNA ladders in hydroxyethyl cellulose, poly(ethyleneoxide) (PEO), and polyethylene glycol and analyzed the effect of polymer concentration, electric field, and temperature of the background electrolyte on the separation performance. Results demonstrated that there was a linear relationship (R=0.942) for 100 to 700bp of DNA and its migration time. Finally, the polymerase chain reaction products of P.g, T.d, and T.f were successfully identified within 8.5 min in 0.5% PEO with uncoated capillary.

Keywords: Capillary electrophoresis; Periodontal pathogens; Polymerase chain reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / genetics
Bacteria / isolation & purification*
Cellulose / chemistry
DNA, Bacterial / isolation & purification
Electrophoresis, Capillary / methods*
Periodontitis / microbiology*
Polyethylene / chemistry*
Polyethylene Glycols / chemistry*
Solutions
Temperature

Substances

DNA, Bacterial
Solutions
Polyethylene Glycols
Polyethylene
Cellulose