Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry

Methods. 2015 Jan 15;72:57-64. doi: 10.1016/j.ymeth.2014.10.033. Epub 2014 Nov 6.


Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.

Keywords: Chromatin; Epigenetics; Histones; Mass spectrometry; Proteomics; SILAC.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatin / chemistry*
  • Epigenomics / methods*
  • Genomics
  • Histones / metabolism
  • Humans
  • Mass Spectrometry / methods*
  • Protein Processing, Post-Translational
  • Proteomics


  • Chromatin
  • Histones