Biochemical characterization of Yarrowia lipolytica LIP8, a secreted lipase with a cleavable C-terminal region

Biochim Biophys Acta. 2015 Feb;1851(2):129-40. doi: 10.1016/j.bbalip.2014.10.012. Epub 2014 Nov 1.


Yarrowia lipolytica is a lipolytic yeast possessing 16 paralog genes coding for lipases. Little information on these lipases has been obtained and only the major secreted lipase, namely YLLIP2, had been biochemically and structurally characterized. Another secreted lipase, YLLIP8, was isolated from Y. lipolytica culture medium and compared with the recombinant enzyme produced in Pichia pastoris. N-terminal sequencing showed that YLLIP8 is produced in its active form after the cleavage of a signal peptide. Mass spectrometry analysis revealed that YLLIP8 recovered from culture medium lacks a C-terminal part of 33 amino acids which are present in the coding sequence. A 3D model of YLLIP8 built from the X-ray structure of the homologous YLLIP2 lipase shows that these truncated amino acids in YLLIP8 belong to an additional C-terminal region predicted to be mainly helical. Western blot analysis shows that YLLIP8 C-tail is rapidly cleaved upon enzyme secretion since both cell-bound and culture supernatant lipases lack this extension. Mature recombinant YLLIP8 displays a true lipase activity on short-, medium- and long-chain triacylglycerols (TAG), with an optimum activity at alkaline pH on medium chain TAG. It has no apparent regioselectivity in TAG hydrolysis, thus generating glycerol and FFAs as final lipolysis products. YLLIP8 properties are distinct from those of the 1,3-regioselective YLLIP2, acting optimally at acidic pH. These lipases are tailored for complementary roles in fatty acid uptake by Y. lipolytica.

Keywords: Enzyme; Lipolysis; Pichia pastoris; Regioselectivity; Signal peptide; Yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bile Acids and Salts / metabolism
  • Crystallography, X-Ray
  • Enzyme Stability
  • Fatty Acids, Nonesterified / metabolism
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Glycerol / metabolism
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Kinetics
  • Lipase / chemistry
  • Lipase / genetics
  • Lipase / metabolism*
  • Lipolysis*
  • Models, Molecular
  • Molecular Sequence Data
  • Olive Oil
  • Pichia / enzymology
  • Pichia / genetics
  • Plant Oils / metabolism
  • Protein Conformation
  • Protein Processing, Post-Translational
  • Protein Sorting Signals
  • Protein Structure, Tertiary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Analysis, Protein
  • Structure-Activity Relationship
  • Substrate Specificity
  • Triglycerides / metabolism
  • Yarrowia / enzymology*
  • Yarrowia / genetics


  • Bile Acids and Salts
  • Fatty Acids, Nonesterified
  • Fungal Proteins
  • Olive Oil
  • Plant Oils
  • Protein Sorting Signals
  • Recombinant Proteins
  • Triglycerides
  • Lipase
  • Glycerol