Isoliquiritigenin attenuates oxidative hepatic damage induced by carbon tetrachloride with or without buthionine sulfoximine

Chem Biol Interact. 2015 Jan 5:225:13-20. doi: 10.1016/j.cbi.2014.10.030. Epub 2014 Nov 6.

Abstract

Glycyrrhizae radix (G. radix) has been demonstrated to have hepatoprotective properties. This study determined the therapeutic effects of isoliquiritigenin (isoLQ) in G. radix, against liver injury induced by CCl4 in rats. CCl4 (0.5 ml/kg/d, twice) or CCl4 plus buthionine sulfoximine exerted severe liver damage assessed by increased plasma levels of alanine aminotransferase and aspartate aminotransferase, in addition to hepatic degeneration and necrosis. These pathological changes were markedly protected by pretreatment with isoLQ (5, 20 mg/kg/d, p.o.) for 3 consecutive days. In addition, pretreatment with isoLQ inhibited CCl4-induced reduction of cytochrome P450 2E1 protein and mRNA expression as well as activity in the liver. Moreover, isoLQ pretreatment reversed the decrease in hepatic antioxidant capacity induced by CCl4 as well as suppressed expression of tumor necrosis factor-alpha and cyclooxigenase-2 in the liver. These results suggest that isoLQ has a protective effect against CCl4-induced liver damage through induction of antioxidant and anti-inflammatory activities.

Keywords: Carbon tetrachloride; Cytochrome P450 2E1; Isoliquiritigenin; Liver; Oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Alanine Transaminase / genetics
  • Animals
  • Aspartate Aminotransferases / blood
  • Aspartate Aminotransferases / genetics
  • Buthionine Sulfoximine / metabolism*
  • Buthionine Sulfoximine / toxicity
  • Carbon Tetrachloride / metabolism*
  • Carbon Tetrachloride / toxicity
  • Chalcones / pharmacology*
  • Chalcones / therapeutic use
  • Chemical and Drug Induced Liver Injury / enzymology
  • Chemical and Drug Induced Liver Injury / metabolism*
  • Cytochrome P-450 CYP2E1 / metabolism*
  • Enzyme Inhibitors / pharmacology*
  • Enzyme Inhibitors / therapeutic use
  • Histocytochemistry
  • Male
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Chalcones
  • Enzyme Inhibitors
  • RNA, Messenger
  • Buthionine Sulfoximine
  • isoliquiritigenin
  • Carbon Tetrachloride
  • Cytochrome P-450 CYP2E1
  • Aspartate Aminotransferases
  • Alanine Transaminase