TRPM7 is required for ovarian cancer cell growth, migration and invasion

Biochem Biophys Res Commun. 2014 Nov 28;454(4):547-53. doi: 10.1016/j.bbrc.2014.10.118. Epub 2014 Oct 30.

Abstract

Our previous study demonstrated that the melastatin-related transient receptor potential channel 7 (TRPM7) was highly expressed in ovarian carcinomas and its overexpression was significantly associated with poor prognosis in ovarian cancer patients. However, the function of TRPM7 in ovarian cancer is mostly unknown. In this study, we examined the roles of TRPM7 in ovarian cancer cell proliferation, migration and invasion. We found that short hairpin RNA interference-mediated silence of TRPM7 significantly inhibited cell proliferation, colony formation, migration and invasion in multiple ovarian cancer cell lines. Mechanistic investigation revealed that silence of TRPM7 decreased phosphorylation levels of Akt, Src and p38 and increased filamentous actin and focal adhesion number in ovarian cancer cells. Thus, our results suggest that TRPM7 is required for proliferation, migration and invasion of ovarian cancer cells through regulating multiple signaling transduction pathways and the formation of focal adhesions.

Keywords: Cell proliferation; Ion channel; Metastasis; Ovarian cancer; TRPM7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Movement*
  • Cell Proliferation*
  • Female
  • HEK293 Cells
  • Humans
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology*
  • Protein-Serine-Threonine Kinases / metabolism*
  • Signal Transduction
  • TRPM Cation Channels / metabolism*

Substances

  • TRPM Cation Channels
  • Protein-Serine-Threonine Kinases
  • TRPM7 protein, human