Evaluation of a simple phenotypic method for the detection of carbapenemase-producing Enterobacteriaceae

Ryoichi Saito; Saho Koyano; Misato Dorin; Yoshimi Higurashi; Yoshiki Misawa; Noriyuki Nagano; Takamasa Kaneko; Kyoji Moriya

doi:10.1016/j.mimet.2014.11.008

Evaluation of a simple phenotypic method for the detection of carbapenemase-producing Enterobacteriaceae

J Microbiol Methods. 2015 Jan:108:45-8. doi: 10.1016/j.mimet.2014.11.008. Epub 2014 Nov 20.

Authors

Ryoichi Saito¹, Saho Koyano², Misato Dorin³, Yoshimi Higurashi⁴, Yoshiki Misawa⁵, Noriyuki Nagano⁶, Takamasa Kaneko⁷, Kyoji Moriya⁸

Affiliations

¹ Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: r-saito.mi@tmd.ac.jp.
² Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: skoyano-tky@umin.ac.jp.
³ Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: rindorindo2@gmail.com.
⁴ Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: yoshimi-tky@umin.ac.jp.
⁵ Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: misawa-tky@umin.org.
⁶ Medical Microbiology Laboratory, Funabashi Municipal Medical Center, Chiba, Japan. Electronic address: naganoyn@d3.dion.ne.jp.
⁷ Kanto Chemical Co., Inc., Tokyo, Japan. Electronic address: kaneko-takamasa@gms.kanto.co.jp.
⁸ Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan. Electronic address: moriya-tky@umin.org.

PMID: 25451461
DOI: 10.1016/j.mimet.2014.11.008

Abstract

We investigated the performance of a phenotypic test, the Carbapenemase Detection Set (MAST-CDS), for the identification of carbapenemase-producing Enterobacteriaceae. Our results indicated that MAST-CDS is rapid, easily performed, simple to interpret, and highly sensitive for the identification of carbapenemase producers, particularly imipenemase producers.

Keywords: Carbapenemase; Enterobacteriaceae; Imipenemase; Klebsiella pneumoniae carbapenemase; New Delhi metallo-β-lactamase; Oxacillinase-48-like.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / analysis*
Bacterial Proteins / metabolism
Enterobacteriaceae / enzymology*
Enterobacteriaceae / isolation & purification
Enterobacteriaceae Infections / microbiology
Enzyme Assays / methods*
Humans
Phenotype
beta-Lactamases / analysis*
beta-Lactamases / metabolism

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase