Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

J Biosci Bioeng. 2015 Apr;119(4):492-5. doi: 10.1016/j.jbiosc.2014.09.018. Epub 2014 Oct 22.


We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments.

Keywords: Biochemical reactions; Cell encapsulation; Centrifuge; Microfluidic device; Monodisperse microdroplets; Plateau–Rayleigh instability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Centrifugation / instrumentation*
  • Glass
  • Microchemistry / instrumentation*
  • Microfluidic Analytical Techniques / instrumentation*
  • Oils*
  • Water*


  • Oils
  • Water