Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

Hitoyoshi Yamashita; Masamune Morita; Haruka Sugiura; Kei Fujiwara; Hiroaki Onoe; Masahiro Takinoue

doi:10.1016/j.jbiosc.2014.09.018

Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

J Biosci Bioeng. 2015 Apr;119(4):492-5. doi: 10.1016/j.jbiosc.2014.09.018. Epub 2014 Oct 22.

Authors

Hitoyoshi Yamashita¹, Masamune Morita², Haruka Sugiura², Kei Fujiwara³, Hiroaki Onoe⁴, Masahiro Takinoue⁵

Affiliations

¹ School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan; Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
² Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
³ Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
⁴ Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
⁵ Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan. Electronic address: takinoue@dis.titech.ac.jp.

PMID: 25454697
DOI: 10.1016/j.jbiosc.2014.09.018

Abstract

We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments.

Keywords: Biochemical reactions; Cell encapsulation; Centrifuge; Microfluidic device; Monodisperse microdroplets; Plateau–Rayleigh instability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Centrifugation / instrumentation*
Glass
Microchemistry / instrumentation*
Microfluidic Analytical Techniques / instrumentation*
Oils*
Water*

Substances

Oils
Water